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Image Search Results
Journal: Chinese Herbal Medicines
Article Title: Sini decoction alleviates inflammation injury after myocardial infarction through regulating arachidonic acid metabolism
doi: 10.1016/j.chmed.2023.12.004
Figure Lengend Snippet: Fig. 6. SND alleviated myocardial inflammation caused by MI through modulating AA metabolism pathway (mean ± SD, n = 5). (A) Expressions of sPLA2, COX-1 and COX-2 in cardiac tissue were measured by Western blot. (B) Expressions of 15-LOX and 5-LOX were measured by Western blot. ##P < 0.01 and ###P < 0.001 vs sham group. *P < 0.05 and **P < 0.01 vs model group.
Article Snippet: The primary antibodies including rabbit monoclonal antibody to 5 Lipoxygenase (ab169755, Abcam, Boston, MA, USA), rabbit monoclonal antibody to 15 Lipoxygenase (ab244205, Abcam, Boston, MA, USA), rabbit monoclonal antibody to NF-kB p65 (ab32536, Abcam, Boston, MA, USA), rabbit monoclonal antibody to COX-1 (ab109025, Abcam, Boston, MA, USA),
Techniques: Western Blot
Journal: Cells
Article Title: Contribution of Oligodendrocytes, Microglia, and Astrocytes to Myelin Debris Uptake in an Explant Model of Inflammatory Demyelination in Rats
doi: 10.3390/cells12172203
Figure Lengend Snippet: Coexpression of Olig2 with LRP1 and MBP in the CA1 region during LPS + IFN-γ-induced demyelination. ( A ) Confocal images displaying coexpression of Olig2 (green) and LRP1 (red) in explants under control conditions and after LPS + IFN-γ treatment for 1 day. Nuclei are stained with DAPI (blue). Arrows point to intensely stained Olig2 + /LRP1 + cells at 1 day. Scale bars: 20 μm. B, left panels, Representative confocal images showing Olig2 (green) and MBP (red) coexpression under control conditions and after LPS + IFN-γ exposure for 1, 2, and 3 days. ( B ) right panels, Higher magnification images displaying representative double-labeled Olig2 + /MBP + cells observed at day 1, day 2, and day 3. Note the accumulation of MBP immunoreactivity at peri-membrane soma sites of Olig2 + cells at day 2 and within the cytosol at day 3. Scale bars: 20 μm. ( C ) Quantitative analysis of double-labeled LRP1 + /Olig2 + cells in slices under control conditions and after LPS + IFN-γ treatment for 1 day. The values represent the mean ± S.E.M. (n = 3). * p < 0.05, LPS + IFN-γ versus control. ( D ) Quantitative analysis of the number of double-labeled Olig2 + /MBP + scored according to somatic MBP immunoreactivity at day 1, day 2, and day 3. The values represent the mean ± S.E.M. (n = 3). * p < 0.05, peri-MBP + versus peri-MBP ++ and cyto-MBP at 1 day; * p < 0.05, peri-MBP ++ versus peri-MBP + and cyto-MBP at 2 days; * p < 0.05, cyto-MBP versus peri-MBP + and peri-MBP ++ at 3 days.
Article Snippet: After fixation and blocking, slices were incubated with the indicated primary antibodies for 48 h at 4 °C: rat monoclonal anti-MBP (1:1000, Merck-Life Science S.r.l., Milan, Italy),
Techniques: Staining, Labeling, Membrane